› 5kkj

X-ray diffraction
2Å resolution

2.0-Angstrom In situ Mylar structure of hen egg-white lysozyme (HEWL) at 293 K

Released:
DOI: 10.2210/pdb5kkj/pdb
PDB entry 5kkj coloured by chain, front view.
PDB entry 5kkj coloured by chain, side view.
PDB entry 5kkj coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
A Versatile System for High-Throughput In Situ X-ray Screening and Data Collection of Soluble and Membrane-Protein Crystals.
Broecker J, Klingel V, Ou WL, Balo AR, Kissick DJ, Ogata CM, Kuo A, Ernst OP
Cryst Growth Des 16 6318-6326 (2016)
PMID: 28261000

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132831 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lysozyme C Chain: A
Molecule details ›
Chain: A
Length: 129 amino acids
Theoretical weight: 14.33 KDa
Source organism: Gallus gallus
UniProt:
  • Canonical: P00698 (Residues: 19-147; Coverage: 100%)
Gene name: LYZ
Sequence domains: C-type lysozyme/alpha-lactalbumin family
Structure domains: Lysozyme

Ligands and Environments

4 bound ligands:
Ligand NA 1 x NA
Ligand CL 2 x CL
Ligand ACY 1 x ACY
Ligand PE5 1 x PE5
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P43212
Unit cell:
a: 79.064Å b: 79.064Å c: 38.336Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.154 0.149 0.2

Quick links

Citations

8 citation in other articles

Crystallogenesis of Membrane Proteins Mediated by Polymer-Bounded Lipid Nanodiscs.
Broecker et al. (2017)
7 more