X-ray diffraction
1.73Å resolution

Crystal structure of the class I human endoplasmic reticulum 1,2-alpha-mannosidase T688A mutant and Thio-disaccharide substrate analog complex


Function and Biology Details

Reaction catalysed:
(1a) Man(9)GlcNAc(2)-[protein] + H(2)O = Man(8)GlcNAc(2)-[protein] (isomer 8A(1,2,3)B(1,2)) + beta-D-mannopyranose
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 455 amino acids
Theoretical weight: 52.27 KDa
Source organism: Homo sapiens
Expression system: Komagataella pastoris
  • Canonical: Q9UKM7 (Residues: 245-699; Coverage: 65%)
Gene names: MAN1B1, UNQ747/PRO1477
Sequence domains: Glycosyl hydrolase family 47
Structure domains: Glycosyltransferase

Ligands and Environments

Carbohydrate polymer : NEW Components: Z5L, MAN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P1
Unit cell:
a: 53.887Å b: 56.302Å c: 89.555Å
α: 105.68° β: 94.15° γ: 114.25°
R R work R free
0.177 0.176 0.209
Expression system: Komagataella pastoris