X-ray diffraction
2.5Å resolution

Crystal structure of Arabidopsis thaliana HCT in complex with p-coumaroyl-CoA


Function and Biology Details

Reaction catalysed:
4-coumaroyl-CoA + shikimate = CoA + 4-coumaroylshikimate
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Shikimate O-hydroxycinnamoyltransferase Chain: A
Molecule details ›
Chain: A
Length: 433 amino acids
Theoretical weight: 48.03 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21(DE3)
  • Canonical: Q9FI78 (Residues: 1-433; Coverage: 100%)
Gene names: At5g48930, HCT, HST, K19E20.4
Sequence domains: Transferase family
Structure domains: Chloramphenicol acetyltransferase-like domain

Ligands and Environments

Cofactor: Ligand WCA 1 x WCA
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P3221
Unit cell:
a: 99.19Å b: 99.19Å c: 84.63Å
α: 90° β: 90° γ: 120°
R R work R free
0.186 0.183 0.243
Expression system: Escherichia coli BL21(DE3)