› 5kj5

X-ray diffraction
2.11Å resolution

Crystal structure of 2-aminomuconate 6-semialdehyde dehydrogenase N169D in complex with NAD+

Released:
DOI: 10.2210/pdb5kj5/pdb
PDB 5kj5 coloured by chain and viewed from the front.
PDB 5kj5 coloured by chain and viewed from the side.
PDB 5kj5 coloured by chain and viewed from the top.
Source organism: Pseudomonas fluorescens
Primary publication:
A Pitcher-and-Catcher Mechanism Drives Endogenous Substrate Isomerization by a Dehydrogenase in Kynurenine Metabolism.
Yang Y, Davis I, Ha U, Wang Y, Shin I, Liu A
J Biol Chem 291 26252-26261 (2016)
PMID: 27810899

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aldehyde dehydrogenase domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 520 amino acids
Theoretical weight: 56.15 KDa
Source organism: Pseudomonas fluorescens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q83V33 (Residues: 1-500; Coverage: 100%)
Gene name: nbaE
Sequence domains: Aldehyde dehydrogenase family
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand NAD 4 x NAD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P212121
Unit cell:
a: 88.684Å b: 142.389Å c: 173.383Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.19 0.236
Expression system: Escherichia coli BL21(DE3)

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Citations

3 citation in other articles

Reassignment of the human aldehyde dehydrogenase ALDH8A1 (ALDH12) to the kynurenine pathway in tryptophan catabolism.
Davis et al. (2018)
2 more

1 mention without citation

A Pitcher-and-Catcher Mechanism Drives Endogenous Substrate Isomerization by a Dehydrogenase in Kynurenine Metabolism.
Yang et al. (2016)