› 5khd

X-ray diffraction
1.75Å resolution

Structure of 1.75 A BldD C-domain-c-di-GMP complex

Released:
DOI: 10.2210/pdb5khd/pdb
PDB 5khd coloured by chain and viewed from the front.
PDB 5khd coloured by chain and viewed from the side.
PDB 5khd coloured by chain and viewed from the top.
Source organism: Streptomyces venezuelae ATCC 10712
Primary publication:
Tetrameric c-di-GMP mediates effective transcription factor dimerization to control Streptomyces development.
Tschowri N, Schumacher MA, Schlimpert S, Chinnam NB, Findlay KC, Brennan RG, Buttner MJ
OpenAccess logo Cell 158 1136-1147 (2014)
PMID: 25171413

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH cro/C1-type domain-containing protein Chains: A, R
Molecule details ›
Chains: A, R
Length: 91 amino acids
Theoretical weight: 10.16 KDa
Source organism: Streptomyces venezuelae ATCC 10712
Expression system: Escherichia coli
UniProt:
  • Canonical: F2RCL8 (Residues: 80-166; Coverage: 52%)
Gene name: SVEN_1089
Structure domains: Arc Repressor Mutant, subunit A

Ligands and Environments

1 bound ligand:
Ligand C2E 4 x C2E
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: C2221
Unit cell:
a: 36.16Å b: 95.58Å c: 99.58Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.183 0.185
Expression system: Escherichia coli

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Citations

28 review citations

Integrating perspectives in actinomycete research: an ActinoBase review of 2020-21.
Undabarrena et al. (2021)
27 more