Structure analysis

Human DNA polymerase eta-DNA ternary complex: reaction first with 1 mM Mn2+ for 1800s then with 5 mM Mn2+ for 60s at 22 degree

X-ray diffraction
1.6Å resolution
Source organism: Homo sapiens
Assembly composition:
hetero trimer (preferred)
Entry contents: 1 distinct polypeptide molecule
2 distinct DNA molecules

Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero trimer
Accessible surface area: 21900 Å2
Buried surface area: 6600 Å2
Dissociation area: 1,400 Å2
Dissociation energy (ΔGdiss): 29 kcal/mol
Dissociation entropy (TΔSdiss): 10 kcal/mol
Interface energy (ΔGint): -52 kcal/mol
Symmetry number: 1

Macromolecules

Chain: A
Length: 435 amino acids
Theoretical weight: 48.62 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9Y253 (Residues: 1-432; Coverage: 61%)
Gene names: POLH, RAD30, RAD30A, XPV
Pfam: impB/mucB/samB family
InterPro:
CATH:

Search similar proteins

Name: DNA (5'-D(*CP*AP*TP*TP*AP*TP*GP*AP*CP*GP*CP*T)-3')
Representative chains: T
Source organism: Homo sapiens [9606]
Expression system: Not provided
Length: 12 nucleotides
Theoretical weight: 3.64 KDa

Search similar DNA

Name: DNA (5'-D(*AP*GP*CP*GP*TP*CP*AP*TP*A)-3')
Representative chains: P
Source organism: Homo sapiens [9606]
Expression system: Not provided
Length: 9 nucleotides
Theoretical weight: 2.74 KDa

Search similar DNA