› 5kf7

X-ray diffraction
1.9Å resolution

Structure of proline utilization A from Sinorhizobium meliloti complexed with L-tetrahydrofuroic acid and NAD+ in space group P3121

Released:
DOI: 10.2210/pdb5kf7/pdb
PDB entry 5kf7 coloured by chain, front view.
PDB entry 5kf7 coloured by chain, side view.
PDB entry 5kf7 coloured by chain, top view.
Source organism: Sinorhizobium meliloti SM11
Primary publication:
Structures of Proline Utilization A (PutA) Reveal the Fold and Functions of the Aldehyde Dehydrogenase Superfamily Domain of Unknown Function.
Luo M, Gamage TT, Arentson BW, Schlasner KN, Becker DF, Tanner JJ
J Biol Chem 291 24065-24075 (2016)
PMID: 27679491

Function and Biology Details

Reactions catalysed: 
L-glutamate 5-semialdehyde + NAD(+) + H(2)O = L-glutamate + NADH
L-proline + a quinone = (S)-1-pyrroline-5-carboxylate + a quinol
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-123681 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bifunctional protein PutA Chain: A
Molecule details ›
Chain: A
Length: 1235 amino acids
Theoretical weight: 131.96 KDa
Source organism: Sinorhizobium meliloti SM11
Expression system: Escherichia coli
UniProt:
  • Canonical: F7X6I3 (Residues: 1-1233; Coverage: 100%)
Gene names: SM11_chr0102, putA
Sequence domains:

Ligands and Environments


Cofactor: Ligand NAD 1 x NAD

Cofactor: Ligand FAD 1 x FAD
6 bound ligands:
Ligand TFB 1 x TFB
Ligand PGE 1 x PGE
Ligand PG4 1 x PG4
Ligand 1PE 2 x 1PE
Ligand SO4 1 x SO4
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 4.2.2
Spacegroup: P3121
Unit cell:
a: 128.843Å b: 128.843Å c: 150.514Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.176 0.174 0.203
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Role of Proline in Pathogen and Host Interactions.
Christgen et al. (2019)
19 other articles

6 mentions without citation

Structure, function, and mechanism of proline utilization A (PutA).
Liu et al. (2017)
5 more