5kel

Electron Microscopy
4.3Å resolution

EBOV GP in complex with variable Fab domains of IgGs c2G4 and c13C6

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
Non-polymer only octadecamer (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (10 distinct):
GP1 Chains: A, E, F
Molecule details ›
Chains: A, E, F
Length: 469 amino acids
Theoretical weight: 50.97 KDa
Source organism: Ebola virus - Mayinga, Zaire, 1976
Expression system: Drosophila melanogaster
UniProt:
  • Canonical: Q05320 (Residues: 33-501; Coverage: 73%)
Gene name: GP
GP2 Chains: B, G, I
Molecule details ›
Chains: B, G, I
Length: 143 amino acids
Theoretical weight: 16.14 KDa
Source organism: Ebola virus - Mayinga, Zaire, 1976
Expression system: Drosophila melanogaster
UniProt:
  • Canonical: Q05320 (Residues: 502-637; Coverage: 21%)
Gene name: GP
c2G4 variable Fab domain heavy chain Chains: H, P, Q
Molecule details ›
Chains: H, P, Q
Length: 120 amino acids
Theoretical weight: 13.57 KDa
Source organism: Homo sapiens
Expression system: Nicotiana benthamiana
c2G4 variable Fab domain light chain Chains: L, T, U
Molecule details ›
Chains: L, T, U
Length: 107 amino acids
Theoretical weight: 11.64 KDa
Source organism: Homo sapiens
Expression system: Nicotiana benthamiana
c13C6 variable Fab domain heavy chain Chains: C, J, M
Molecule details ›
Chains: C, J, M
Length: 121 amino acids
Theoretical weight: 13.17 KDa
Source organism: Homo sapiens
Expression system: Nicotiana benthamiana
c13C6 variable Fab domain light chain Chains: D, N, O
Molecule details ›
Chains: D, N, O
Length: 107 amino acids
Theoretical weight: 11.61 KDa
Source organism: Homo sapiens
Expression system: Nicotiana benthamiana

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.3Å
Relevant EMDB volumes: EMD-8240
Expression systems:
  • Drosophila melanogaster
  • Nicotiana benthamiana