PDBe 5keg

X-ray diffraction
2.2Å resolution

Crystal structure of APOBEC3A in complex with a single-stranded DNA


Function and Biology Details

Reaction catalysed:
Cytosine in single-stranded DNA + H(2)O = uracil in single-stranded DNA + NH(3)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
DNA dC->dU-editing enzyme APOBEC-3A Chain: A
Molecule details ›
Chain: A
Length: 203 amino acids
Theoretical weight: 23.3 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
  • Canonical: P31941 (Residues: 1-199; Coverage: 100%)
Gene name: APOBEC3A
Sequence domains: APOBEC-like C-terminal domain
DNA (5'-D(*TP*TP*CP*TP*T)-3') Chain: B
Molecule details ›
Chain: B
Length: 15 nucleotides
Theoretical weight: 4.5 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: I222
Unit cell:
a: 56.583Å b: 72.666Å c: 114.971Å
α: 90° β: 90° γ: 90°
R R work R free
0.177 0.177 0.225
Expression systems:
  • Escherichia coli
  • Not provided