5kdu

X-ray diffraction
2Å resolution

ZmpB metallopeptidase in complex with a2,6-Sialyl T-antigen

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Peptidase M60 domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 530 amino acids
Theoretical weight: 60.08 KDa
Source organism: Clostridium perfringens ATCC 13124
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: A0A0H2YN38 (Residues: 497-1003; Coverage: 31%)
Gene name: CPF_1489
Sequence domains: Peptidase M60, enhancin and enhancin-like

Ligands and Environments

Carbohydrate polymer : NEW Components: A2G, GAL, SIA
3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P212121
Unit cell:
a: 66.14Å b: 68.38Å c: 170.94Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.17 0.169 0.201
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'