› 5kcy

X-ray diffraction
2.3Å resolution

Crystal structure of the aromatic prenyltransferase AtaPT from Aspergillus terreus A8-4 in complex with geranyl S-thiolodiphosphate and (+)-butyrolactone II

Released:
DOI: 10.2210/pdb5kcy/pdb
PDB 5kcy coloured by chain and viewed from the front.
PDB 5kcy coloured by chain and viewed from the side.
PDB 5kcy coloured by chain and viewed from the top.
Source organism: Aspergillus terreus
Primary publication:
Molecular insights into the enzyme promiscuity of an aromatic prenyltransferase.
Chen R, Gao B, Liu X, Ruan F, Zhang Y, Lou J, Feng K, Wunsch C, Li SM, Dai J, Sun F
Nat Chem Biol 13 226-234 (2017)
PMID: 27992881

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aromatic prenyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 419 amino acids
Theoretical weight: 46.9 KDa
Source organism: Aspergillus terreus
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A1B0UHJ4 (Residues: 11-424; Coverage: 98%)
Gene name: PT
Sequence domains: Tryptophan dimethylallyltransferase

Ligands and Environments

2 bound ligands:
Ligand XBU 2 x XBU
Ligand GST 2 x GST
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P21212
Unit cell:
a: 96.95Å b: 134.91Å c: 68.69Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.204 0.246
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Xanthone Biosynthetic Pathway in Plants: A Review.
Remali et al. (2022)
7 more