X-ray diffraction
2.15Å resolution

Crystal structure of iron-sulfur cluster containing photolyase PhrB mutant I51W


Function and Biology Details

Reaction catalysed:
(6-4) photoproduct (in DNA) = 2 pyrimidine residues (in DNA)
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
(6-4) photolyase Chains: A, B
Molecule details ›
Chains: A, B
Length: 518 amino acids
Theoretical weight: 59.3 KDa
Source organism: Agrobacterium fabrum str. C58
Expression system: Escherichia coli
  • Canonical: A9CH39 (Residues: 1-507; Coverage: 100%)
Gene names: Atu4765, phrB
Sequence domains: Deoxyribodipyrimidine photo-lyase-related protein
Structure domains:

Ligands and Environments

Cofactor: Ligand FAD 2 x FAD
2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P212121
Unit cell:
a: 97.607Å b: 107.243Å c: 113.672Å
α: 90° β: 90° γ: 90°
R R work R free
0.166 0.164 0.213
Expression system: Escherichia coli