5kck

X-ray diffraction
2.2Å resolution

Crystal structure of anthranilate synthase component I from Streptococcus pneumoniae TIGR4

Released:
Entry authors: Chang C, Michalska K, Bigelow L, Jedrzejczak R, ANDERSON WF, JOACHIMIAK A, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed:
(1a) L-glutamine + H(2)O = L-glutamate + NH(3) 
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Anthranilate synthase component 1 Chain: A
Molecule details ›
Chain: A
Length: 450 amino acids
Theoretical weight: 51.52 KDa
Source organism: Streptococcus pneumoniae TIGR4
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: A0A0H2URN1 (Residues: 2-448; Coverage: 99%)
Gene names: SP_1817, trpE
Sequence domains:
Structure domains: Anthranilate synthase

Ligands and Environments

1 bound ligand:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2
Unit cell:
a: 120.946Å b: 86.127Å c: 55.792Å
α: 90° β: 93.45° γ: 90°
R-values:
R R work R free
0.184 0.182 0.224
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'