› 5kce

X-ray diffraction
1.85Å resolution

Crystal Structure of the ER-alpha Ligand-binding Domain (Y537S) in Complex with an N-methyl, 2-chlorobenzyl OBHS-N derivative

Released:
DOI: 10.2210/pdb5kce/pdb
PDB 5kce coloured by chain and viewed from the front.
PDB 5kce coloured by chain and viewed from the side.
PDB 5kce coloured by chain and viewed from the top.
Source organism: Homo sapiens
Primary publication:
Full antagonism of the estrogen receptor without a prototypical ligand side chain.
Srinivasan S, Nwachukwu JC, Bruno NE, Dharmarajan V, Goswami D, Kastrati I, Novick S, Nowak J, Cavett V, Zhou HB, Boonmuen N, Zhao Y, Min J, Frasor J, Katzenellenbogen BS, Griffin PR, Katzenellenbogen JA, Nettles KW
Nat. Chem. Biol. 13 111-118 (2017)
PMID: 27870835

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Estrogen receptor Chains: A, B
Molecule details ›
Chains: A, B
Length: 257 amino acids
Theoretical weight: 29.3 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P03372 (Residues: 298-554; Coverage: 43%)
Gene names: ESR, ESR1, NR3A1
Sequence domains: Ligand-binding domain of nuclear hormone receptor
Structure domains: Retinoid X Receptor
Nuclear receptor coactivator 2 Chains: C, D
Molecule details ›
Chains: C, D
Length: 13 amino acids
Theoretical weight: 1.58 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q15596 (Residues: 686-698; Coverage: 1%)
Gene names: BHLHE75, NCOA2, SRC2, TIF2

Ligands and Environments

1 bound ligand:
Ligand OB3 3 x OB3
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P21
Unit cell:
a: 52.577Å b: 75.326Å c: 57.996Å
α: 90° β: 110.82° γ: 90°
R-values:
R R work R free
0.195 0.192 0.25
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

4 review citations

Recent progress in selective estrogen receptor downregulators (SERDs) for the treatment of breast cancer.
Shagufta et al. (2020)
3 more

1 mention without citation

Pocket similarity identifies selective estrogen receptor modulators as microtubule modulators at the taxane site.
Lo et al. (2019)