5kc4

X-ray diffraction
3.4Å resolution

Structure of TmRibU, orthorhombic crystal form

Released:
Source organism: Thermotoga maritima MSB8
Entry authors: Karpowich NK, Wang DN, Song JM

Function and Biology Details

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Riboflavin transporter RibU Chains: A, E
Molecule details ›
Chains: A, E
Length: 181 amino acids
Theoretical weight: 19.8 KDa
Source organism: Thermotoga maritima MSB8
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9X1G6 (Residues: 2-173; Coverage: 94%)
Gene names: TM_1455, ribU
Sequence domains: ECF transporter, substrate-specific component
Structure domains: Arp2/3 complex 21 kDa subunit ARPC3

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P212121
Unit cell:
a: 65.769Å b: 80.784Å c: 118.028Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.25 0.249 0.27
Expression system: Escherichia coli BL21(DE3)