5kbj

X-ray diffraction
3.09Å resolution

Structure of Rep-DNA complex

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
hetero hexamer (preferred)
homo tetramer
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Replication initiator A, N-terminal Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 132 amino acids
Theoretical weight: 15.97 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: D2JDC3 (Residues: 2-133; Coverage: 41%)
Gene names: SAP042A_013, SAP058A_012, SAP071A_014
Sequence domains: Replication initiator protein A (RepA) N-terminus
DNA (32-MER) Chain: R
Molecule details ›
Chain: R
Length: 32 nucleotides
Theoretical weight: 9.84 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (32-MER) Chain: W
Molecule details ›
Chain: W
Length: 32 nucleotides
Theoretical weight: 9.84 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P1
Unit cell:
a: 59.2Å b: 77.5Å c: 100.7Å
α: 80.8° β: 75.5° γ: 71.9°
R-values:
R R work R free
0.231 0.224 0.277
Expression systems:
  • Escherichia coli
  • Not provided