PDBe 5kbd

X-ray diffraction
2.8Å resolution

Structural Studies of Transcription Factor p73 DNA Binding Domain Bound to PA26 20-mer Response Element

Released:
Source organism: Homo sapiens
Entry authors: Nguyen HT, Huang D

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Tumor protein p73 Chains: A, B
Molecule details ›
Chains: A, B
Length: 207 amino acids
Theoretical weight: 23.45 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O15350 (Residues: 115-312; Coverage: 31%)
Gene names: P73, TP73
Sequence domains: P53 DNA-binding domain
Structure domains: Immunoglobulin-like
DNA (5'-D(P*GP*GP*AP*CP*AP*AP*GP*TP*CP*T)-3') Chain: C
Molecule details ›
Chain: C
Length: 10 nucleotides
Theoretical weight: 3.07 KDa
Source organism: Homo sapiens
Expression system: Not provided
DNA (5'-D(P*AP*GP*AP*CP*TP*TP*GP*TP*CP*C)-3') Chain: D
Molecule details ›
Chain: D
Length: 10 nucleotides
Theoretical weight: 3.02 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P61
Unit cell:
a: 174.878Å b: 174.878Å c: 34.282Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.282 0.279 0.308
Expression systems:
  • Escherichia coli
  • Not provided