X-ray diffraction
1.7Å resolution

Crystal structure of human dihydroorotate dehydrogenase at 1.7 A resolution

Source organism: Homo sapiens
Entry authors: Lewis TA, Sykes DB, Law JM, Munoz B, Scadden DT, Rustiguel JK, Nonato MC, Schreiber SL

Function and Biology Details

Reaction catalysed:
(S)-dihydroorotate + a quinone = orotate + a quinol
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Dihydroorotate dehydrogenase (quinone), mitochondrial Chain: A
Molecule details ›
Chain: A
Length: 368 amino acids
Theoretical weight: 39.94 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
  • Canonical: Q02127 (Residues: 29-395; Coverage: 93%)
Gene name: DHODH
Sequence domains: Dihydroorotate dehydrogenase
Structure domains: Aldolase class I

Ligands and Environments

Cofactor: Ligand FNR 1 x FNR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: LNLS BEAMLINE W01B-MX2
Spacegroup: P3221
Unit cell:
a: 90.14Å b: 90.14Å c: 122.152Å
α: 90° β: 90° γ: 120°
R R work R free
0.138 0.136 0.16
Expression system: Escherichia coli