› 5k8h

X-ray diffraction
1.07Å resolution

The X-ray crystal structure of a parallel poly(rA) double helix generated by rA7 at acidic pH

Released:
DOI: 10.2210/pdb5k8h/pdb
PDB entry 5k8h coloured by chain, front view.
PDB entry 5k8h coloured by chain, side view.
PDB entry 5k8h coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
Crystal structure of a poly(rA) staggered zipper at acidic pH: evidence that adenine N1 protonation mediates parallel double helix formation.
Gleghorn ML, Zhao J, Turner DH, Maquat LE
Nucleic Acids Res 44 8417-24 (2016)
PMID: 27288442

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-196937 (preferred)
Entry contents:
1 distinct RNA molecule
Macromolecule:
RNA 7-mer Chain: A
Molecule details ›
Chain: A
Length: 7 nucleotides
Theoretical weight: 2.26 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand NH4 1 x NH4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P42212
Unit cell:
a: 21.253Å b: 21.253Å c: 14.946Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.106 0.106 0.108
Expression system: Not provided

Quick links

Citations

1 review citation

Parallel-stranded DNA and RNA duplexes - structural features and potential applications.
Szabat et al. (2017)
8 other articles

2 mentions without citation

Crystal structure of a poly(rA) staggered zipper at acidic pH: evidence that adenine N1 protonation mediates parallel double helix formation.
Gleghorn et al. (2016)
1 more