5k77

X-ray diffraction
2.17Å resolution

Dbr1 in complex with 7-mer branched RNA

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct RNA molecules
Macromolecules (3 distinct):
Metallophos domain-containing protein Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 360 amino acids
Theoretical weight: 41.85 KDa
Source organism: Entamoeba histolytica
Expression system: Escherichia coli
UniProt:
  • Canonical: C4M1P9 (Residues: 1-354; Coverage: 100%)
Gene name: EHI_062730
Sequence domains: Calcineurin-like phosphoesterase
branch 1 of branched RNA 5'-UAA(-2'GU)CA-3' Chains: V, W, X, Y, Z
Molecule details ›
Chains: V, W, X, Y, Z
Length: 5 nucleotides
Theoretical weight: 1.55 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided
brnch 2 of branched RNA 5'-UAA(-2'GU)CA-3' Chains: v, w, x, y, z
Molecule details ›
Chains: v, w, x, y, z
Length: 2 nucleotides
Theoretical weight: 606 Da
Source organism: Saccharomyces cerevisiae
Expression system: Not provided

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P212121
Unit cell:
a: 73.151Å b: 141.892Å c: 212.511Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.182 0.223
Expression systems:
  • Escherichia coli
  • Not provided