› 5k5w

X-ray diffraction
2.59Å resolution

Crystal structure of limiting CO2-inducible protein LCIB

Released:
DOI: 10.2210/pdb5k5w/pdb
PDB entry 5k5w coloured by chain, front view.
PDB entry 5k5w coloured by chain, side view.
PDB entry 5k5w coloured by chain, top view.
Source organism: Chlamydomonas reinhardtii
Primary publication:
Structural insights into the LCIB protein family reveals a new group of β-carbonic anhydrases.
Jin S, Sun J, Wunder T, Tang D, Cousins AB, Sze SK, Mueller-Cajar O, Gao YG
Proc Natl Acad Sci U S A 113 14716-14721 (2016)
PMID: 27911826

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171203 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Limiting CO2-inducible protein B/C beta carbonyic anhydrase domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 297 amino acids
Theoretical weight: 31.78 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Escherichia coli
UniProt:
  • Canonical: Q0ZAI6 (Residues: 53-346; Coverage: 66%)
Gene names: CHLRE_10g452800v5, Pmp1
Sequence domains: Limiting CO2-inducible proteins B/C beta carbonyic anhydrases

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P212121
Unit cell:
a: 43.835Å b: 80.218Å c: 143.753Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.269 0.268 0.272
Expression system: Escherichia coli

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Citations

18 review citations

Progress and challenges of engineering a biophysical CO2-concentrating mechanism into higher plants.
Rae et al. (2017)
17 more

1 mention without citation

Structural insights into the LCIB protein family reveals a new group of β-carbonic anhydrases.
Jin et al. (2016)