PDBe 5k25

X-ray diffraction
3.05Å resolution

Crystal structure of human phosphatase PRL-2 in complex with the ADP-bound Bateman domain of human magnesium transporter CNNM3

Released:

Function and Biology Details

Reaction catalysed:
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Cellular component:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Protein tyrosine phosphatase type IVA 2 Chain: A
Molecule details ›
Chain: A
Length: 190 amino acids
Theoretical weight: 21.93 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q12974 (Residues: 1-167; Coverage: 100%)
Gene names: BM-008, PRL2, PTP4A2, PTPCAAX2
Sequence domains: Protein-tyrosine phosphatase
Structure domains: Protein tyrosine phosphatase superfamily
Metal transporter CNNM3 Chain: C
Molecule details ›
Chain: C
Length: 156 amino acids
Theoretical weight: 17.76 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q8NE01 (Residues: 309-452; Coverage: 20%)
Gene names: ACDP3, CNNM3
Sequence domains: CBS domain
Structure domains: CBS-domain

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Spacegroup: I222
Unit cell:
a: 52.152Å b: 124.401Å c: 164.305Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.234 0.232 0.283
Expression system: Escherichia coli BL21