5k18

X-ray diffraction
2.73Å resolution

The NatB Acetyltransferase Complex Bound To bisubstrate inhibitor

Released:

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Uncharacterized protein Chains: A, C
Molecule details ›
Chains: A, C
Length: 766 amino acids
Theoretical weight: 89.47 KDa
Source organism: Candida albicans WO-1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: C4YFL7 (Residues: 1-745; Coverage: 100%)
Gene name: CAWG_01335
Sequence domains: N-acetyltransferase B complex (NatB) non catalytic subunit
N-acetyltransferase domain-containing protein Chains: B, D
Molecule details ›
Chains: B, D
Length: 188 amino acids
Theoretical weight: 22 KDa
Source organism: Candida albicans WO-1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: C4YDZ9 (Residues: 1-188; Coverage: 100%)
Gene name: CAWG_00748
Sequence domains: Acetyltransferase (GNAT) family
Bisubstrate inhibitor Chains: E, F
Molecule details ›
Chains: E, F
Length: 11 amino acids
Theoretical weight: 1.16 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17B1
Spacegroup: P1
Unit cell:
a: 83.054Å b: 91.087Å c: 101.74Å
α: 70.12° β: 88.91° γ: 84.05°
R-values:
R R work R free
0.189 0.187 0.249
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided