› 5k0a

X-ray diffraction
1.71Å resolution

Structure of an oxidoreductase from Synechocystis sp. PCC6803

Released:
DOI: 10.2210/pdb5k0a/pdb
PDB 5k0a coloured by chain and viewed from the front.
PDB 5k0a coloured by chain and viewed from the side.
PDB 5k0a coloured by chain and viewed from the top.
Source organism: Synechocystis sp. PCC 6803 substr. Kazusa
Primary publication:
Unprecedented pathway of reducing equivalents in a diflavin-linked disulfide oxidoreductase.
Buey RM, Arellano JB, López-Maury L, Galindo-Trigo S, Velázquez-Campoy A, Revuelta JL, de Pereda JM, Florencio FJ, Schürmann P, Buchanan BB, Balsera M
Proc Natl Acad Sci U S A 114 12725-12730 (2017)
PMID: 29133410

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pyr_redox_2 domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 330 amino acids
Theoretical weight: 36.46 KDa
Source organism: Synechocystis sp. PCC 6803 substr. Kazusa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P74746 (Residues: 2-326; Coverage: 100%)
Gene name: slr0600
Sequence domains: Pyridine nucleotide-disulphide oxidoreductase

Ligands and Environments

6 bound ligands:
Ligand FAD 8 x FAD
Ligand NO3 8 x NO3
Ligand PG4 6 x PG4
Ligand 1PE 5 x 1PE
Ligand PO4 2 x PO4
Ligand PGE 1 x PGE
1 modified residue:
Ligand CSO 4 x CSO

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P21
Unit cell:
a: 66.029Å b: 128.43Å c: 92.728Å
α: 90° β: 91.61° γ: 90°
R-values:
R R work R free
0.166 0.165 0.193
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Exploring the Diversity of the Thioredoxin Systems in Cyanobacteria.
Mallén-Ponce et al. (2022)
1 more