› 5jye

X-ray diffraction
2.23Å resolution

Structures of Streptococcus agalactiae GBS GAPDH in different enzymatic states

Released:
DOI: 10.2210/pdb5jye/pdb
PDB 5jye coloured by chain and viewed from the front.
PDB 5jye coloured by chain and viewed from the side.
PDB 5jye coloured by chain and viewed from the top.
Source organism: Streptococcus agalactiae
Primary publication:
Crystal Structures of Group B Streptococcus Glyceraldehyde-3-Phosphate Dehydrogenase: Apo-Form, Binary and Ternary Complexes.
Schormann N, Ayres CA, Fry A, Green TJ, Banerjee S, Ulett GC, Chattopadhyay D
OpenAccess logo PLoS One 11 e0165917 (2016)
PMID: 27875551

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glyceraldehyde-3-phosphate dehydrogenase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 356 amino acids
Theoretical weight: 38.21 KDa
Source organism: Streptococcus agalactiae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9ALW2 (Residues: 1-336; Coverage: 100%)
Gene names: AX245_09885, C4618_13560, C6N07_02390, DK41_09330, GE200_01450, NCTC6175_00390, NCTC8183_01962, RDF_1710, TH70_1537, gap, gapA
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand NAD 2 x NAD
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P21
Unit cell:
a: 78.247Å b: 107.358Å c: 87.904Å
α: 90° β: 113.01° γ: 90°
R-values:
R R work R free
0.209 0.207 0.249
Expression system: Escherichia coli

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Citations

6 citation in other articles

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