› 5jyc

X-ray diffraction
2Å resolution

Crystal structure of the E153Q mutant of the CFTR inhibitory factor Cif containing the adducted 14,15-EET hydrolysis intermediate

Released:
DOI: 10.2210/pdb5jyc/pdb
PDB 5jyc coloured by chain and viewed from the front.
PDB 5jyc coloured by chain and viewed from the side.
PDB 5jyc coloured by chain and viewed from the top.
Source organism: Pseudomonas aeruginosa UCBPP-PA14
Primary publication:
Pseudomonas aeruginosa sabotages the generation of host proresolving lipid mediators.
Flitter BA, Hvorecny KL, Ono E, Eddens T, Yang J, Kwak DH, Bahl CD, Hampton TH, Morisseau C, Hammock BD, Liu X, Lee JS, Kolls JK, Levy BD, Madden DR, Bomberger JM
Proc. Natl. Acad. Sci. U.S.A. 114 136-141 (2017)
PMID: 27980032

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
AB hydrolase-1 domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 301 amino acids
Theoretical weight: 34.16 KDa
Source organism: Pseudomonas aeruginosa UCBPP-PA14
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0H2ZD27 (Residues: 25-319; Coverage: 100%)
Gene name: PA14_26090
Sequence domains: Alpha/beta hydrolase family
Structure domains: alpha/beta hydrolase

Ligands and Environments

1 bound ligand:
Ligand EET 4 x EET
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL14-1
Spacegroup: C2
Unit cell:
a: 168.93Å b: 83.602Å c: 89.241Å
α: 90° β: 100.38° γ: 90°
R-values:
R R work R free
0.155 0.153 0.191
Expression system: Escherichia coli

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Citations

19 review citations

Specialized pro-resolving mediators in respiratory diseases.
Cagnina et al. (2022)
18 more