X-ray diffraction
1.88Å resolution

A low magnesium structure of the isochorismate synthase, EntC

Source organism: Escherichia coli O157:H7
Primary publication:
An Open and Shut Case: The Interaction of Magnesium with MST Enzymes.
OpenAccess logo J Am Chem Soc 138 9277-93 (2016)
PMID: 27373320

Function and Biology Details

Reaction catalysed:
Chorismate = isochorismate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Isochorismate synthase EntC Chains: A, B
Molecule details ›
Chains: A, B
Length: 391 amino acids
Theoretical weight: 42.98 KDa
Source organism: Escherichia coli O157:H7
Expression system: Escherichia coli BL21(DE3)
  • Canonical: P0AEJ3 (Residues: 1-391; Coverage: 100%)
Gene names: ECs0632, Z0735, entC
Sequence domains: chorismate binding enzyme
Structure domains: Anthranilate synthase

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P41212
Unit cell:
a: 80.205Å b: 80.205Å c: 272.2Å
α: 90° β: 90° γ: 90°
R R work R free
0.188 0.187 0.229
Expression system: Escherichia coli BL21(DE3)