› 5jvt

X-ray diffraction
3.1Å resolution

Crystal structure of the DNA binding domain of transcription factor FLI1 in complex with an 11-mer DNA GACCGGAAGTG

Released:
DOI: 10.2210/pdb5jvt/pdb
PDB entry 5jvt coloured by chain, front view.
PDB entry 5jvt coloured by chain, side view.
PDB entry 5jvt coloured by chain, top view.
Source organisms:
Primary publication:
Structures of mithramycin analogues bound to DNA and implications for targeting transcription factor FLI1.
Hou C, Weidenbach S, Cano KE, Wang Z, Mitra P, Ivanov DN, Rohr J, Tsodikov OV
Nucleic Acids Res 44 8990-9004 (2016)
PMID: 27587584

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-118881 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Friend leukemia integration 1 transcription factor Chains: A, D, G
Molecule details ›
Chains: A, D, G
Length: 104 amino acids
Theoretical weight: 12.1 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q01543 (Residues: 276-375; Coverage: 22%)
Gene name: FLI1
Sequence domains: Ets-domain
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
DNA (5'-D(*GP*AP*CP*CP*GP*GP*AP*AP*GP*TP*G)-3') Chains: B, E, H
Molecule details ›
Chains: B, E, H
Length: 11 nucleotides
Theoretical weight: 3.42 KDa
Source organism: Endothia gyrosa
Expression system: Not provided
DNA (5'-D(*CP*AP*CP*TP*TP*CP*CP*GP*GP*TP*C)-3') Chains: C, F, I
Molecule details ›
Chains: C, F, I
Length: 11 nucleotides
Theoretical weight: 3.29 KDa
Source organism: Endothia gyrosa
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand PO4 1 x PO4
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P6122
Unit cell:
a: 191.845Å b: 191.845Å c: 87.556Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.196 0.194 0.237
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

4 review citations

Targeting Transcription Factors for Cancer Treatment.
Lambert et al. (2018)
3 more

5 mentions without citation

Structures of mithramycin analogues bound to DNA and implications for targeting transcription factor FLI1.
Hou et al. (2016)
4 more