5jvt

X-ray diffraction
3.1Å resolution

Crystal structure of the DNA binding domain of transcription factor FLI1 in complex with an 11-mer DNA GACCGGAAGTG

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Friend leukemia integration 1 transcription factor Chains: A, D, G
Molecule details ›
Chains: A, D, G
Length: 104 amino acids
Theoretical weight: 12.1 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q01543 (Residues: 276-375; Coverage: 22%)
Gene name: FLI1
Sequence domains: Ets-domain
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
DNA (5'-D(*GP*AP*CP*CP*GP*GP*AP*AP*GP*TP*G)-3') Chains: B, E, H
Molecule details ›
Chains: B, E, H
Length: 11 nucleotides
Theoretical weight: 3.42 KDa
Source organism: Endothia gyrosa
Expression system: Not provided
DNA (5'-D(*CP*AP*CP*TP*TP*CP*CP*GP*GP*TP*C)-3') Chains: C, F, I
Molecule details ›
Chains: C, F, I
Length: 11 nucleotides
Theoretical weight: 3.29 KDa
Source organism: Endothia gyrosa
Expression system: Not provided

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P6122
Unit cell:
a: 191.845Å b: 191.845Å c: 87.556Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.196 0.194 0.237
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided