Electron Microscopy
4.1Å resolution

Active human apoptosome with procaspase-9

Source organisms:
Related structures: EMD-8178

Function and Biology Details

Reaction catalysed:
Strict requirement for an Asp residue at position P1 and with a marked preference for His at position P2. It has a preferred cleavage sequence of Leu-Gly-His-Asp-|-Xaa
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
Non-polymer only octadecamer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Apoptotic protease-activating factor 1 Chains: A, B, C, D, E, F, G
Molecule details ›
Chains: A, B, C, D, E, F, G
Length: 1248 amino acids
Theoretical weight: 142.02 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
  • Canonical: O14727 (Residues: 1-1248; Coverage: 100%)
Gene names: APAF1, KIAA0413
Sequence domains:
Cytochrome c Chains: H, I, J, K, L, M, N
Molecule details ›
Chains: H, I, J, K, L, M, N
Length: 104 amino acids
Theoretical weight: 11.6 KDa
Source organism: Bos taurus
  • Canonical: P62894 (Residues: 2-105; Coverage: 99%)
Gene names: CYC, CYCS
Sequence domains: Cytochrome c
Caspase-9 subunit p35 Chains: O, P, Q, R
Molecule details ›
Chains: O, P, Q, R
Length: 95 amino acids
Theoretical weight: 11.14 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
  • Canonical: P55211 (Residues: 1-95; Coverage: 23%)
Gene names: CASP9, MCH6
Sequence domains: Caspase recruitment domain

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.1Å
Relevant EMDB volumes: EMD-8178
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli 'BL21-Gold(DE3)pLysS AG'