› 5js4

X-ray diffraction
1.48Å resolution

Crystal structure of phiAB6 tailspike

Released:
DOI: 10.2210/pdb5js4/pdb
PDB entry 5js4 coloured by chain, front view.
PDB entry 5js4 coloured by chain, side view.
PDB entry 5js4 coloured by chain, top view.
Source organism: Bacteriophage sp.
Primary publication:
Structural basis for fragmenting the exopolysaccharide of Acinetobacter baumannii by bacteriophage ΦAB6 tailspike protein.
Lee IM, Tu IF, Yang FL, Ko TP, Liao JH, Lin NT, Wu CY, Ren CT, Wang AH, Chang CM, Huang KF, Wu SH
Sci Rep 7 42711 (2017)
PMID: 28209973

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-103047 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pectate lyase superfamily protein domain-containing protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 719 amino acids
Theoretical weight: 78.42 KDa
Source organism: Bacteriophage sp.
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A159BDB5 (Residues: 1-699; Coverage: 100%)
Gene name: phiAB6_gp40
Sequence domains: Pectate lyase superfamily protein

Ligands and Environments

1 bound ligand:
Ligand MLA 9 x MLA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL15A1
Spacegroup: C2
Unit cell:
a: 135.018Å b: 77.98Å c: 248.08Å
α: 90° β: 100.46° γ: 90°
R-values:
R R work R free
0.137 0.136 0.156
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Reprogramming bacteriophage host range: design principles and strategies for engineering receptor binding proteins.
Dunne et al. (2021)
4 more

3 mentions without citation

Structural basis for fragmenting the exopolysaccharide of Acinetobacter baumannii by bacteriophage ΦAB6 tailspike protein.
Lee et al. (2017)
2 more