X-ray diffraction
2Å resolution

X-ray structure of furin in complex with the inhibitory antibody Nb14


Function and Biology Details

Reaction catalysed:
Release of mature proteins from their proproteins by cleavage of -Arg-Xaa-Yaa-Arg-|- bonds, where Xaa can be any amino acid and Yaa is Arg or Lys. Releases albumin, complement component C3 and von Willebrand factor from their respective precursors.
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Furin Chains: A, B
Molecule details ›
Chains: A, B
Length: 482 amino acids
Theoretical weight: 52.39 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
  • Canonical: P09958 (Residues: 108-574; Coverage: 61%)
Gene names: FUR, FURIN, PACE, PCSK3
Sequence domains:
Structure domains:
camelid VHH fragment Chains: C, D
Molecule details ›
Chains: C, D
Length: 117 amino acids
Theoretical weight: 12.62 KDa
Source organism: Camelus dromedarius
Expression system: Escherichia coli
Structure domains: Immunoglobulins
CMK-inhibitor Chains: G, H
Molecule details ›
Chains: G, H
Length: 6 amino acids
Theoretical weight: 749 Da
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Unit cell:
a: 169.775Å b: 50.039Å c: 144.244Å
α: 90° β: 90° γ: 90°
R R work R free
0.165 0.163 0.197
Expression systems:
  • Homo sapiens
  • Escherichia coli
  • Not provided