› 5jmd

X-ray diffraction
2.4Å resolution

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Heparin-sulfate lyase N-terminal domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 655 amino acids
Theoretical weight: 76.44 KDa
Source organism: Bacteroides thetaiotaomicron VPI-5482
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q89YS4 (Residues: 23-666; Coverage: 97%)
Gene name: BT_4657
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand MG 1 x MG
1 modified residue:
Ligand MLY 1 x MLY

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P21
Unit cell:
a: 66.359Å b: 80.412Å c: 83.543Å
α: 90° β: 104.08° γ: 90°
R-values:
R R work R free
0.221 0.219 0.258
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Marine Polysaccharides: Occurrence, Enzymatic Degradation and Utilization.
Bäumgen et al. (2021)
8 other articles

2 mentions without citation

A novel ulvan lyase family with broad-spectrum activity from the ulvan utilisation loci of Formosa agariphila KMM 3901.
Konasani et al. (2018)
1 more