X-ray diffraction
2Å resolution

Receptor binding domain of Botulinum neurotoxin A in complex with human glycosylated SV2C


Function and Biology Details

Reaction catalysed:
Limited hydrolysis of proteins of the neuroexocytosis apparatus, synaptobrevin (also known as neuronal vesicle-associated membrane protein, VAMP), synaptosome-associated protein of 25 kDa (SNAP25) or syntaxin. No detected action on small molecule substrates.
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (4 distinct):
Botulinum neurotoxin A heavy chain Chains: A, B
Molecule details ›
Chains: A, B
Length: 428 amino acids
Theoretical weight: 49.88 KDa
Source organism: Clostridium botulinum
Expression system: Escherichia coli
  • Canonical: P0DPI1 (Residues: 872-1296; Coverage: 33%)
Gene names: CBO0806, CLC_0862, bna, botA
Sequence domains:
Structure domains:
Synaptic vesicle glycoprotein 2C Chains: C, D
Molecule details ›
Chains: C, D
Length: 117 amino acids
Theoretical weight: 13.59 KDa
Source organism: Homo sapiens
Expression system: Mammalian expression vector pCBio
  • Canonical: Q496J9 (Residues: 473-567; Coverage: 13%)
Gene names: KIAA1054, SV2C
Structure domains: E3 ubiquitin-protein ligase SopA

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, FUC
Carbohydrate polymer : NEW Components: NAG, FUC
3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: C2
Unit cell:
a: 109.002Å b: 111.853Å c: 126.253Å
α: 90° β: 101.29° γ: 90°
R R work R free
0.178 0.176 0.216
Expression systems:
  • Escherichia coli
  • Mammalian expression vector pCBio