5jlh

Electron Microscopy
3.9Å resolution

Cryo-EM structure of a human cytoplasmic actomyosin complex at near-atomic resolution

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero undecamer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Actin, cytoplasmic 2 Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 374 amino acids
Theoretical weight: 41.71 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63261 (Residues: 2-375; Coverage: 100%)
Gene names: ACTG, ACTG1
Sequence domains: Actin
Structure domains:
Alpha-actinin A; Myosin-14 Chains: F, G
Molecule details ›
Chains: F, G
Length: 1039 amino acids
Theoretical weight: 117.57 KDa
Source organisms: Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P05095 (Residues: 265-502; Coverage: 28%)
  • Canonical: Q7Z406 (Residues: 1-799; Coverage: 40%)
Gene names: DDB_G0268632, FP17425, KIAA2034, MYH14, abpA, actnA
Sequence domains:
Tropomyosin alpha-3 chain Chains: H, I, J, K
Molecule details ›
Chains: H, I, J, K
Length: 135 amino acids
Theoretical weight: 11.51 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.9Å
Relevant EMDB volumes: EMD-8164
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli