5ji0

X-ray diffraction
1.98Å resolution

PPARgamma-RXRalpha(S427F) heterodimer in complex with SRC-1, rosiglitazone, and 9-cis-retanoic acid

Released:
Source organism: Homo sapiens
Entry authors: Bloudoff K, Larsen NA

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero tetramer (preferred)
hetero dimer
PDBe Complex ID:
PDB-CPX-148713 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Retinoic acid receptor RXR-alpha Chain: A
Molecule details ›
Chain: A
Length: 242 amino acids
Theoretical weight: 27.1 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P19793 (Residues: 223-462; Coverage: 52%)
Gene names: NR2B1, RXRA
Sequence domains: Ligand-binding domain of nuclear hormone receptor
Structure domains: Retinoid X Receptor
Peroxisome proliferator-activated receptor gamma Chain: D
Molecule details ›
Chain: D
Length: 273 amino acids
Theoretical weight: 31.15 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P37231 (Residues: 234-505; Coverage: 54%)
Gene names: NR1C3, PPARG
Sequence domains: Ligand-binding domain of nuclear hormone receptor
Structure domains: Retinoid X Receptor
Nuclear receptor coactivator 1 Chains: E, F
Molecule details ›
Chains: E, F
Length: 27 amino acids
Theoretical weight: 2.83 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q15788 (Residues: 676-700; Coverage: 2%)
Gene names: BHLHE74, NCOA1, SRC1
Sequence domains: Steroid receptor coactivator

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P212121
Unit cell:
a: 53.88Å b: 66.527Å c: 165.927Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.192 0.246
Expression systems:
  • Escherichia coli
  • Not provided