PDBe 5jhh

X-ray diffraction
2.3Å resolution

Crystal structure of the ternary complex between the human RhoA, its inhibitor and the DH/PH domain of human ARHGEF11

Released:
Source organism: Homo sapiens
Entry authors: Lv Z, Wang R, Ma L, Miao Q, Wu J, Yan Z, Li J, Miao L, Wang F

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Rho guanine nucleotide exchange factor 11 Chains: A, E
Molecule details ›
Chains: A, E
Length: 369 amino acids
Theoretical weight: 42.91 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O15085 (Residues: 714-1081; Coverage: 24%)
Gene names: ARHGEF11, KIAA0380
Sequence domains:
Structure domains:
Transforming protein RhoA Chains: B, F
Molecule details ›
Chains: B, F
Length: 181 amino acids
Theoretical weight: 20.47 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P61586 (Residues: 1-181; Coverage: 94%)
Gene names: ARH12, ARHA, RHO12, RHOA
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E SUPERBRIGHT
Spacegroup: P21
Unit cell:
a: 88.855Å b: 119.049Å c: 90.526Å
α: 90° β: 114.39° γ: 90°
R-values:
R R work R free
0.196 0.193 0.239
Expression system: Escherichia coli BL21(DE3)