› 5jgu

X-ray diffraction
1.47Å resolution

Spin-Labeled T4 Lysozyme Construct R119V1

Released:
DOI: 10.2210/pdb5jgu/pdb
PDB entry 5jgu coloured by chain, front view.
PDB entry 5jgu coloured by chain, side view.
PDB entry 5jgu coloured by chain, top view.
Source organism: Escherichia phage T4
Primary publication:
Toward Precise Interpretation of DEER-Based Distance Distributions: Insights from Structural Characterization of V1 Spin-Labeled Side Chains.
Balo AR, Feyrer H, Ernst OP
Biochemistry 55 5256-63 (2016)
PMID: 27532325

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133020 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endolysin Chain: A
Molecule details ›
Chain: A
Length: 164 amino acids
Theoretical weight: 18.57 KDa
Source organism: Escherichia phage T4
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P00720 (Residues: 1-164; Coverage: 100%)
Gene name: E
Sequence domains: Phage lysozyme

Ligands and Environments

4 bound ligands:
Ligand PO4 1 x PO4
Ligand CL 3 x CL
Ligand K 1 x K
Ligand V1A 1 x V1A
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P3221
Unit cell:
a: 59.919Å b: 59.919Å c: 96.154Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.153 0.151 0.175
Expression system: Escherichia coli BL21(DE3)

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Citations

3 review citations

Site-Directed Spin Labeling EPR for Studying Membrane Proteins.
Sahu et al. (2018)
2 more