5j9t

X-ray diffraction
2.7Å resolution

Crystal structure of the NuA4 core complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Histone acetyltransferase ESA1 Chains: A, E, I
Molecule details ›
Chains: A, E, I
Length: 305 amino acids
Theoretical weight: 36.56 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: Q08649 (Residues: 141-445; Coverage: 69%)
Gene names: ESA1, O5257, YOR244W
Sequence domains:
Chromatin modification-related protein EAF6 Chains: B, F, J
Molecule details ›
Chains: B, F, J
Length: 113 amino acids
Theoretical weight: 12.92 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P47128 (Residues: 1-113; Coverage: 100%)
Gene names: EAF6, J1854, YJR082C
Sequence domains: Histone acetyltransferase subunit NuA4
Enhancer of polycomb-like protein 1 Chains: C, G, K
Molecule details ›
Chains: C, G, K
Length: 280 amino acids
Theoretical weight: 33.32 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P43572 (Residues: 121-400; Coverage: 34%)
Gene names: EPL1, YFL024C
Chromatin modification-related protein YNG2 Chains: D, H, L
Molecule details ›
Chains: D, H, L
Length: 120 amino acids
Theoretical weight: 13.83 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P38806 (Residues: 1-120; Coverage: 43%)
Gene names: EAF4, NBN1, YHR090C, YNG2
Sequence domains: Inhibitor of growth proteins N-terminal histone-binding

Ligands and Environments

No bound ligands
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P21
Unit cell:
a: 86.685Å b: 137.582Å c: 168.076Å
α: 90° β: 97.63° γ: 90°
R-values:
R R work R free
0.188 0.187 0.219
Expression system: Escherichia coli