› 5j4i

X-ray diffraction
2.21Å resolution

Crystal Structure of the L-arginine/agmatine antiporter from E. coli at 2.2 Angstroem resolution

Released:
DOI: 10.2210/pdb5j4i/pdb
PDB entry 5j4i coloured by chain, front view.
PDB entry 5j4i coloured by chain, side view.
PDB entry 5j4i coloured by chain, top view.
Source organism: Escherichia coli O157:H7
Primary publication:
Insights into the molecular basis for substrate binding and specificity of the wild-type L-arginine/agmatine antiporter AdiC.
Ilgü H, Jeckelmann JM, Gapsys V, Ucurum Z, de Groot BL, Fotiadis D
Proc Natl Acad Sci U S A 113 10358-63 (2016)
PMID: 27582465

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157970 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Arginine/agmatine antiporter Chains: A, B
Molecule details ›
Chains: A, B
Length: 453 amino acids
Theoretical weight: 47.84 KDa
Source organism: Escherichia coli O157:H7
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P60061 (Residues: 1-445; Coverage: 100%)
Gene names: JW4076, adiC, aniC, b4115, yjdD, yjdE
Sequence domains: Amino acid permease

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21212
Unit cell:
a: 104.739Å b: 175.424Å c: 73.148Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.197 0.21
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

9 review citations

The Human SLC7A5 (LAT1): The Intriguing Histidine/Large Neutral Amino Acid Transporter and Its Relevance to Human Health.
Scalise et al. (2018)
8 more

13 mentions without citation

Insights into the Structure, Function, and Ligand Discovery of the Large Neutral Amino Acid Transporter 1, LAT1.
Singh et al. (2018)
12 more