5j3e

X-ray diffraction
2.6Å resolution

Crystal Structure of Human THYN1 protein in complex with 5-methylcytosine containing DNA

Released:
Source organism: Homo sapiens
Entry authors: Halabelian L, Tempel W, Li Y, Bountra C, Edwards AM, Arrowsmith CH, Structural Genomics Consortium (SGC)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Thymocyte nuclear protein 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 225 amino acids
Theoretical weight: 25.74 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9P016 (Residues: 1-225; Coverage: 100%)
Gene names: HSPC144, MDS012, My0054, THY28, THYN1
Sequence domains: EVE domain
Structure domains: ph1033 like domains
5-methylcytosine containing DNA Chains: C, D, E, F
Molecule details ›
Chains: C, D, E, F
Length: 12 nucleotides
Theoretical weight: 3.68 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 40.327Å b: 73.232Å c: 108.201Å
α: 90° β: 91.18° γ: 90°
R-values:
R R work R free
0.211 0.209 0.249
Expression systems:
  • Escherichia coli
  • Not provided