PDBe 5j28

X-ray diffraction
2Å resolution

Ki67-PP1g (protein phosphatase 1, gamma isoform) holoenzyme complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Serine/threonine-protein phosphatase PP1-gamma catalytic subunit Chains: A, B
Molecule details ›
Chains: A, B
Length: 305 amino acids
Theoretical weight: 35.04 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P36873 (Residues: 7-308; Coverage: 94%)
Gene name: PPP1CC
Sequence domains:
Structure domains: Purple Acid Phosphatase; chain A, domain 2
Proliferation marker protein Ki-67 Chains: C, D
Molecule details ›
Chains: C, D
Length: 46 amino acids
Theoretical weight: 5.19 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P46013 (Residues: 496-536; Coverage: 1%)
Gene name: MKI67

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P61
Unit cell:
a: 90.832Å b: 90.832Å c: 206.717Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.159 0.154 0.197
Expression system: Escherichia coli