5j13

X-ray diffraction
2.3Å resolution

Structural basis for TSLP antagonism by the therapeutic antibody Tezepelumab (MEDI9929 / AMG-157)

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Thymic stromal lymphopoietin Chain: A
Molecule details ›
Chain: A
Length: 147 amino acids
Theoretical weight: 16.52 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q969D9 (Residues: 8-8, 29-159; Coverage: 81%)
  • Best match: Q969D9-2 (Residues: 1-63)
Gene name: TSLP
Sequence domains: Thymic stromal lymphopoietin
anti-TSLP Fab-fragment, light chain Chain: B
Molecule details ›
Chain: B
Length: 245 amino acids
Theoretical weight: 25.87 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
anti-TSLP Fab-fragment, heavy chain Chain: C
Molecule details ›
Chain: C
Length: 263 amino acids
Theoretical weight: 28.44 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 2
Spacegroup: P3212
Unit cell:
a: 51.726Å b: 51.726Å c: 370.028Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.19 0.189 0.215
Expression systems:
  • Escherichia coli BL21(DE3)
  • Homo sapiens