5j11

X-ray diffraction
2.56Å resolution

Structure of human TSLP in complex with TSLPR and IL-7Ralpha

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
Non-polymer only trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Thymic stromal lymphopoietin Chain: A
Molecule details ›
Chain: A
Length: 147 amino acids
Theoretical weight: 16.52 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q969D9 (Residues: 8-8, 29-159; Coverage: 81%)
  • Best match: Q969D9-2 (Residues: 1-63)
Gene name: TSLP
Sequence domains: Thymic stromal lymphopoietin
Interleukin-7 receptor subunit alpha Chain: B
Molecule details ›
Chain: B
Length: 240 amino acids
Theoretical weight: 27.59 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P16871 (Residues: 21-236; Coverage: 49%)
Gene name: IL7R
Sequence domains:
Structure domains:
Cytokine receptor-like factor 2 Chain: C
Molecule details ›
Chain: C
Length: 230 amino acids
Theoretical weight: 26.57 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9HC73 (Residues: 1-221; Coverage: 57%)
  • Best match: Q9HC73-3 (Residues: 1-109)
Gene names: CRL2, CRLF2, ILXR, TSLPR
Structure domains: Immunoglobulins

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 2
Spacegroup: C2
Unit cell:
a: 135.783Å b: 66.644Å c: 91.971Å
α: 90° β: 109.2° γ: 90°
R-values:
R R work R free
0.193 0.191 0.218
Expression systems:
  • Escherichia coli BL21(DE3)
  • Homo sapiens