› 5iwn

X-ray diffraction
3.75Å resolution

Bacterial sodium channel pore domain, high bromide

Released:
DOI: 10.2210/pdb5iwn/pdb
PDB entry 5iwn coloured by chain, front view.
PDB entry 5iwn coloured by chain, side view.
PDB entry 5iwn coloured by chain, top view.
Source organism: Alkalilimnicola ehrlichii
Primary publication:
Unfolding of a Temperature-Sensitive Domain Controls Voltage-Gated Channel Activation.
Arrigoni C, Rohaim A, Shaya D, Findeisen F, Stein RA, Nurva SR, Mishra S, Mchaourab HS, Minor DL
Cell 164 922-36 (2016)
PMID: 26919429

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170847 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ion transport domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 152 amino acids
Theoretical weight: 17.46 KDa
Source organism: Alkalilimnicola ehrlichii
Expression system: Escherichia coli
UniProt:
  • Canonical: Q0ABW0 (Residues: 143-288; Coverage: 51%)
Gene name: Mlg_0322
Sequence domains: Ion transport protein
Structure domains: Helix Hairpins

Ligands and Environments

1 bound ligand:
Ligand BR 2 x BR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: I222
Unit cell:
a: 157.4Å b: 160.87Å c: 167.35Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.231 0.228 0.28
Expression system: Escherichia coli

Quick links

Citations

9 review citations

New Structures and Gating of Voltage-Dependent Potassium (Kv) Channels and Their Relatives: A Multi-Domain and Dynamic Question.
Barros et al. (2019)
8 more