PDBe 5ivx

X-ray diffraction
2.1Å resolution

Crystal Structure of B4.2.3 T-Cell Receptor and H2-Dd P18-I10 Complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
H-2 class I histocompatibility antigen, D-D alpha chain Chain: A
Molecule details ›
Chain: A
Length: 277 amino acids
Theoretical weight: 32.27 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01900 (Residues: 26-301; Coverage: 81%)
Gene name: H2-D1
Sequence domains: Immunoglobulin C1-set domain
Structure domains:
Beta-2-microglobulin Chain: B
Molecule details ›
Chain: B
Length: 100 amino acids
Theoretical weight: 11.79 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01887 (Residues: 21-119; Coverage: 100%)
Gene name: B2m
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
T-CELL RECEPTOR ALPHA CHAIN Chain: E
Molecule details ›
Chain: E
Length: 194 amino acids
Theoretical weight: 21.66 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
Structure domains: Immunoglobulins
T-CELL RECEPTOR BETA CHAIN Chain: F
Molecule details ›
Chain: F
Length: 234 amino acids
Theoretical weight: 26.84 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Surface protein gp120 Chain: P
Molecule details ›
Chain: P
Length: 10 amino acids
Theoretical weight: 1.08 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P04578 (Residues: 311-320; Coverage: 1%)
Gene name: env

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: C2
Unit cell:
a: 210.107Å b: 51.316Å c: 93.741Å
α: 90° β: 97.14° γ: 90°
R-values:
R R work R free
0.182 0.18 0.22
Expression systems:
  • Escherichia coli BL21(DE3)
  • Escherichia coli
  • Not provided