› 5iv1

Solution NMR

Solution Structure of DNA Dodecamer with 8-oxoguanine at 4th Position

Released:
DOI: 10.2210/pdb5iv1/pdb
PDB entry 5iv1 coloured by chain, ensemble of 14 models, front view.
PDB entry 5iv1 coloured by chain, ensemble of 14 models, side view.
PDB entry 5iv1 coloured by chain, ensemble of 14 models, top view.
Source organism: synthetic construct
Primary publication:
8-Oxoguanine Affects DNA Backbone Conformation in the EcoRI Recognition Site and Inhibits Its Cleavage by the Enzyme.
Hoppins JJ, Gruber DR, Miears HL, Kiryutin AS, Kasymov RD, Petrova DV, Endutkin AV, Popov AV, Yurkovskaya AV, Fedechkin SO, Brockerman JA, Zharkov DO, Smirnov SL
PLoS One 11 e0164424 (2016)
PMID: 27749894

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-118809 (preferred)
Entry contents:
1 distinct DNA molecule
Macromolecule:
DNA (5'-D(*CP*GP*CP*(8OG)P*AP*AP*TP*TP*CP*GP*CP*G)-3') Chains: A, B
Molecule details ›
Chains: A, B
Length: 12 nucleotides
Theoretical weight: 3.68 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand 8OG 2 x 8OG

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 37%
Refinement method: simulated annealing
Expression system: Not provided

Quick links

Citations

1 review citation

Beyond the Lesion: Back to High Fidelity DNA Synthesis.
Kaszubowski et al. (2021)
5 other articles

3 mentions without citation

8-Oxoguanine Affects DNA Backbone Conformation in the EcoRI Recognition Site and Inhibits Its Cleavage by the Enzyme.
Hoppins et al. (2016)
2 more