› 5iv0

X-ray diffraction
2.1Å resolution

Crystal structure of Eis from Mycobacterium tuberculosis in complex with sulfonamide inhibitor 39 and coenzyme A

Released:
DOI: 10.2210/pdb5iv0/pdb
PDB entry 5iv0 coloured by chain, front view.
PDB entry 5iv0 coloured by chain, side view.
PDB entry 5iv0 coloured by chain, top view.
Source organism: Mycobacterium tuberculosis CDC1551
Primary publication:
Sulfonamide-Based Inhibitors of Aminoglycoside Acetyltransferase Eis Abolish Resistance to Kanamycin in Mycobacterium tuberculosis.
Garzan A, Willby MJ, Green KD, Gajadeera CS, Hou C, Tsodikov OV, Posey JE, Garneau-Tsodikova S
J Med Chem 59 10619-10628 (2016)
PMID: 27933949

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161420 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
N-acetyltransferase Eis Chain: A
Molecule details ›
Chain: A
Length: 428 amino acids
Theoretical weight: 46.66 KDa
Source organism: Mycobacterium tuberculosis CDC1551
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P9WFK7 (Residues: 1-402; Coverage: 100%)
Gene names: MTCY253.04, Rv2416c, eis
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand COA 1 x COA
Ligand 6E9 1 x 6E9
Ligand CL 3 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: R32
Unit cell:
a: 174.8Å b: 174.8Å c: 121.985Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.193 0.192 0.219
Expression system: Escherichia coli BL21(DE3)

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Citations

6 review citations

The Role of Antibiotic-Target-Modifying and Antibiotic-Modifying Enzymes in Mycobacterium abscessus Drug Resistance.
Luthra et al. (2018)
5 more

2 mentions without citation

Sulfonamide-Based Inhibitors of Aminoglycoside Acetyltransferase Eis Abolish Resistance to Kanamycin in Mycobacterium tuberculosis.
Garzan et al. (2016)
1 more