› 5iuy

X-ray diffraction
2.29Å resolution

Structural insights of the outer-membrane channel OprN

Released:
DOI: 10.2210/pdb5iuy/pdb
PDB entry 5iuy coloured by chain, front view.
PDB entry 5iuy coloured by chain, side view.
PDB entry 5iuy coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
Xenon for tunnelling analysis of the efflux pump component OprN.
Ntsogo Enguéné YV, Phan G, Garnier C, Ducruix A, Prangé T, Broutin I
PLoS One 12 e0184045 (2017)
PMID: 28886086

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191253 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Multidrug efflux outer membrane protein OprN Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 453 amino acids
Theoretical weight: 49.54 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9I0Y7 (Residues: 26-472; Coverage: 100%)
Gene names: PA2495, oprN
Sequence domains: Outer membrane efflux protein
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand PLM 5 x PLM
Ligand CL 1 x CL
Ligand BOG 3 x BOG
Ligand NA 1 x NA
Ligand FMT 1 x FMT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: I4
Unit cell:
a: 257.648Å b: 257.648Å c: 81.511Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.188 0.225
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Structure, Assembly, and Function of Tripartite Efflux and Type 1 Secretion Systems in Gram-Negative Bacteria.
Alav et al. (2021)
2 other articles

3 mentions without citation

Xenon for tunnelling analysis of the efflux pump component OprN.
Ntsogo Enguéné et al. (2017)
2 more