X-ray diffraction
2.46Å resolution

Crystal Structure of Human NEIL1(P2G) bound to duplex DNA containing THF

Source organisms:

Function and Biology Details

Reaction catalysed:
The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Endonuclease 8-like 1 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 400 amino acids
Theoretical weight: 44.93 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
  • Canonical: Q96FI4 (Residues: 1-390; Coverage: 100%)
Gene name: NEIL1
Sequence domains:
Structure domains:
DNA (26-MER) Chains: D, E
Molecule details ›
Chains: D, E
Length: 26 nucleotides
Theoretical weight: 7.95 KDa
Source organism: unidentified
Expression system: Not provided
DNA (26-MER) Chain: F
Molecule details ›
Chain: F
Length: 26 nucleotides
Theoretical weight: 7.97 KDa
Source organism: unidentified
Expression system: Not provided

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P212121
Unit cell:
a: 73.306Å b: 109.036Å c: 169.837Å
α: 90° β: 90° γ: 90°
R R work R free
0.213 0.211 0.263
Expression systems:
  • Escherichia coli
  • Not provided