› 5ish

X-ray diffraction
2.15Å resolution

Crystal structure of mouse CARM1 in complex with inhibitor SA0765

Released:
DOI: 10.2210/pdb5ish/pdb
PDB entry 5ish coloured by chain, front view.
PDB entry 5ish coloured by chain, side view.
PDB entry 5ish coloured by chain, top view.
Source organism: Mus musculus
Entry authors: Cura V, Marechal N, Mailliot J, Troffer-Charlier N, Hassenboehler P, Wurtz JM, Bonnefond L, Cavarelli J

Function and Biology Details

Reaction catalysed: 
(1a) S-adenosyl-L-methionine + [protein]-L-arginine = S-adenosyl-L-homocysteine + [protein]-N(omega)-methyl-L-arginine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194407 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Histone-arginine methyltransferase CARM1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 361 amino acids
Theoretical weight: 40.85 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9WVG6 (Residues: 130-487; Coverage: 59%)
Gene names: Carm1, Prmt4
Sequence domains: Ribosomal protein L11 methyltransferase (PrmA)
Structure domains:

Ligands and Environments

6 bound ligands:
Ligand 765 4 x 765
Ligand EDO 8 x EDO
Ligand PEG 4 x PEG
Ligand DXE 6 x DXE
Ligand M2M 1 x M2M
Ligand PG4 1 x PG4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P21212
Unit cell:
a: 74.675Å b: 98.413Å c: 206.846Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.166 0.164 0.198
Expression system: Spodoptera frugiperda

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